FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb289.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb289.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45800314
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC10490742.290538881458324TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC1739830.3798729414824536TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC1237380.27016845343025375TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC972330.21229767114697073TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC761900.16635257129459854TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2996100.057.88449
CGGAAGA3028950.057.7229654
TCGGAAG3081000.056.866093
AGAGCAC3063750.056.666728
ATCGGAA3103150.056.502082
GATCGGA3162100.055.4850161
GAAGAGC3196900.054.5076076
AAGAGCA3318100.052.507627
GGAAGAG3585400.048.7921875
TCGTATG1196550.044.9830242-43
ATGCCGT1235500.044.6116846-47
TCTCGTA1328300.044.52500540-41
CTCGTAT1319100.044.1664142-43
CGTATGC1263250.043.97773444-45
GCCGTCT1270150.043.96328448-49
TATGCCG1266150.043.74003246-47
AATCTCG1790050.043.53732738-39
TGCCGTC1386550.043.3259348-49
ATCTCGT1546300.043.19394740-41
CGTCTTC1357300.043.15609750-51
Produced by FastQC (version 0.11.5)