FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb288.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb288.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48805476
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1241870.2544530044128655TruSeq Adapter, Index 3 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT471300.015.21513550-51
CGTCTTC479550.014.58189850-51
TGCCGTC500100.014.41343348-49
ATGCCGT493150.014.211884546-47
TCGTATG453100.014.18846942-43
GCCGTCT498500.014.1165548-49
TATGCCG510600.014.07515846-47
CTCGTAT471150.013.97770442-43
GCTTGAA525800.013.60897858-59
CGTATGC545000.013.10826544-45
TCTCGTA498250.012.94209740-41
AAGGGGG578000.012.64649366-67
TGCTTGA579900.012.58918858-59
AGGGGGG509800.012.36784666-67
ATCTCGT540900.012.29937740-41
GTATGCC622700.011.40776644-45
AAAAGGG654400.011.31248564-65
CATCTCG594600.010.89685138-39
AAAGGGG665800.09.63842564-65
CTTGAAA805050.09.44331860-61
Produced by FastQC (version 0.11.5)