FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb287.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb287.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48067548
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC15337283.190776446512312TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGC1071890.22299660469471003TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3344700.052.180284
GAGCACA3308350.052.1705259
TCGGAAG3398050.051.5517233
ATCGGAA3422750.051.3045462
AGAGCAC3390250.050.9970788
GATCGGA3466900.050.669431
GAAGAGC3521200.049.293896
AAGAGCA3654750.047.4506387
GGAAGAG3928500.044.3836445
CTCGTAT1732700.044.38063442-43
CGTATGC1836100.044.25369644-45
TATGCCG1844350.044.03899846-47
TCGTATG1695000.044.02643242-43
TGCCGTC1846750.043.87370348-49
CCGTCTT1824400.043.76023550-51
ATGCCGT1816350.043.58126446-47
TCTCGTA1745450.043.52571540-41
ATCTCGT1803600.043.22498740-41
CGATCTC1846550.043.20256438-39
GCCGTCT1798050.043.172648-49
Produced by FastQC (version 0.11.5)