FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb282.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb282.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23927483
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41550.022.69946548-49
TATCGCG10500.021.94863940-41
CGTATGC57700.016.42833746-47
CCGTATC63400.015.25205938-39
TCCGTAT62350.014.97543536-37
CGCGTAT17350.014.65147444-45
ATCGCGT17750.014.18724842-43
TCGTATG48400.013.94023244-45
TATCTCG50950.013.19676340-41
CGTATCG22300.011.61301538-39
ATGCCGT83100.011.43549948-49
GTTCCGT83300.011.23512534-35
AGTTCCG86100.010.98010634-35
GTATGCC88000.010.96074146-47
CTCGTAT67150.010.86148944-45
GTCCGGT44250.010.4135381
GTCCGTT46650.010.1832921
CGTATCT66950.010.11387638-39
GTCCGGA53200.010.090361
TTCCGTA102550.09.80004436-37
Produced by FastQC (version 0.11.5)