FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23960668
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38150.013.5756648-49
GTCCGGT45850.011.9148031
CCCGTAT30150.011.65930751
GTCCGTT44300.011.3665981
GTCCGGA55650.09.9019611
CGTATGC54250.09.85330246-47
GTCCGCT59450.09.5886561
CGCGTAT15700.09.07926444-45
CTCCGCT104800.09.0656111
GTCCGCA53200.08.83999351
TCGTATG46650.08.81035644-45
GTCCGAT58750.08.73261451
GTCCCGT55850.08.590661
GTCCGAA41050.08.5634141
GTCCGTA27300.08.5263231
GTTCGGT39150.08.4936551
GTCCGGG79450.08.3707151
GTCGAAA29250.08.1203091
GTCCATT145850.08.04486751
GTCCAAT81550.07.92215441
Produced by FastQC (version 0.11.5)