FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb280.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb280.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19278838
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33100.019.3067646-47
TCGTATG36800.017.30087942-43
CGTATGC46500.013.74309344-45
CTCGTAT51550.011.705442-43
AATCTCG59100.011.69864938-39
GTCCGTA19700.011.5752211
GTATGCC65750.011.02019444-45
AATCGCG12150.010.55960538-39
ATGCCGT68000.010.48087446-47
GTCGGAT28150.09.9569851
CCCGTAT25800.09.7591151
TCTCGTA70900.09.31603240-41
GTCGCAT19200.09.1549241
GTCGCAA14600.09.1108621
GCCGTCT83350.08.75019848-49
AGATCGG471200.08.47203394-95
GATCGGA444950.08.31002994-95
GCCCGAT39550.08.28813651
GCCGTTT41300.08.2820281
GTCCGGT38800.08.0810261
Produced by FastQC (version 0.11.5)