FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19877983
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31800.013.9711246-47
CGTATTA11250.010.5552793
TCGTATG40150.010.473659542-43
CGTATGC47550.010.14291544-45
GTCCGTT35050.010.0298321
GTCGGAT28750.09.9143311
GTCGCAT20850.09.7974361
GTCCGCT48350.09.3342011
GTCCGGA46950.08.6006911
TAGATCG220300.08.31094994-95
CCCGTAT26450.08.2619421
GTCCGCA45600.08.2302351
GTCGCGA8158.275261E-68.1605691
GTCCGGT33850.08.1399041
CTCGTAT52850.08.13662442-43
CACACGT336500.08.02391194-95
ATCGGAA802050.08.0148994-95
GATCGGA867500.07.998738394-95
TTAACCG19050.07.97803167
GTCCAAT71050.07.9566981
Produced by FastQC (version 0.11.5)