FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20250676
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29050.014.31141446-47
GTCCGGT36450.010.0353441
TCGTATG37650.09.96959142-43
GTCCGTT39100.09.9626811
CGTATGC43550.09.87372444-45
GTCGCAA13600.09.4311341
GTTCGTT32850.09.3997671
TATCTCG39400.09.04548438-39
CCCGTAT26950.08.9898091
TATCGCG6953.6379788E-128.54657238-39
GATCGGA657800.08.50914394-95
GTCCGCA48100.08.394851
ATCGGAA607350.08.15634194-95
TAGATCG149850.08.09812194-95
GCCCGAT37850.08.0325451
GTCGTAT23100.08.020321
GTCCGGA46200.08.020321
AGATCGG614450.08.0195894-95
GTCCGTA21650.07.89921051
GTCCGCT49150.07.8288971
Produced by FastQC (version 0.11.5)