FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24630192
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT44750.012.8452291
GTCCGTT47100.012.60777951
GTCCGGA55850.010.8026441
TATGCCG29800.010.68284746-47
GTCCGCT67500.010.2753971
CCCGTAT30400.09.5324771
GTCCGAA41200.09.45511
GTCGGAT37250.09.4374561
GTCGCAT27850.09.2112391
GTCCGTA26000.08.9530751
GTCCGCA57500.08.9228881
CGTATTA11959.458745E-118.7445463
GTCGGTT52800.08.6374751
GTCCAAT89000.08.4870471
GTCACAT198300.08.4567111
GATCGGA903000.08.17625994-95
AGATCGG975100.08.04903894-95
ATCGGAA835800.08.02955494-95
GTCCAGT206750.07.927261
GTCGGAA60000.07.9176861
Produced by FastQC (version 0.11.5)