FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb276.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb276.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19104576
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27900.014.56115944-45
TCGTATG35500.012.84904142-43
CCCGTAT24500.012.7977461
GTCCGTT37450.012.051121
CGTATTA10050.011.8157833
GTCCCGT45500.010.8586921
GTCCGGT37450.010.7825811
GTCCGGA44700.010.6279151
CGTATGC41350.010.57158642-43
CCGTATT28150.010.12445452
GTCCGTA22800.09.7930641
CCCCGCT74450.09.6991671
GTCCGCT48650.09.5697111
GTATGCC60750.09.50307344-45
CTCCGGT71750.09.1371931
GTCGGAT28700.09.1040871
CTCCGAT56500.09.0809421
CTCCCGT59950.08.9545741
GTCGCAT20700.08.9505531
CTCCGCT85600.08.8797721
Produced by FastQC (version 0.11.5)