FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22440873
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT28450.013.6927831
GTCCGTT39450.011.9219791
GTCCGGT39900.011.0731261
CGTATTA12450.011.0637333
CGCTAGT12600.010.9342161
GTCCGAA37650.010.8515971
TATGCCG27900.010.21830546-47
CTCCGCT99450.09.6973131
GTCCGGA50450.09.605041
CCCGAAT50450.09.1342041
CCGTATT32800.08.9787542
GTCCGCT58250.08.9713331
GTCCTAT50550.08.8341921
GTCCGTA26700.08.7185641
GTCGCGA8251.0498225E-68.6376881
GTCCGCA51400.08.5956751
GTTCCGT44250.08.5888861
CCCGTTT59100.08.5207641
GTCGCAT23600.08.4546851
ATCGGAA744500.08.34437694-95
Produced by FastQC (version 0.11.5)