FastQCFastQC Report
Fri 2 Aug 2024
HKMCHBGXV_n01_SRS_S2_WT_S4_Rep1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMCHBGXV_n01_SRS_S2_WT_S4_Rep1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6580547
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC374590.569238393100148RNA PCR Primer, Index 4 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC136780.20785506128897796RNA PCR Primer, Index 4 (100% over 44bp)
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTAT95650.1453526583732325RNA PCR Primer, Index 4 (100% over 48bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC91030.13833196541260173RNA PCR Primer, Index 4 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC88890.13507995611914936RNA PCR Primer, Index 4 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG81840.1243665610168881RNA PCR Primer, Index 4 (100% over 49bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC80420.12220868569132626RNA PCR Primer, Index 4 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC77710.11809048700662728RNA PCR Primer, Index 4 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)