FastQCFastQC Report
Fri 2 Aug 2024
HKMCHBGXV_n01_SRS_S2_WT_S3_Rep1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMCHBGXV_n01_SRS_S2_WT_S3_Rep1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5548535
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC228470.4117663491354024RNA PCR Primer, Index 3 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC88380.15928528881948117RNA PCR Primer, Index 3 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC62120.11195748066832056RNA PCR Primer, Index 3 (100% over 44bp)
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTAT59080.10647855695242077RNA PCR Primer, Index 3 (100% over 48bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC57910.10436989223281462RNA PCR Primer, Index 3 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)