FastQCFastQC Report
Fri 2 Aug 2024
HKMCHBGXV_n01_SRS_S2_WT_S1_Rep1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMCHBGXV_n01_SRS_S2_WT_S1_Rep1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7842337
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC518790.6615247470237507RNA PCR Primer, Index 1 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC189380.24148413923043605RNA PCR Primer, Index 1 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC131660.16788362958643577RNA PCR Primer, Index 1 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC126730.1615972381702036RNA PCR Primer, Index 1 (100% over 44bp)
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTAT120350.15346190810213844RNA PCR Primer, Index 1 (100% over 48bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC114690.1462446717094662RNA PCR Primer, Index 1 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC111950.1427508152225542RNA PCR Primer, Index 1 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG103210.13160617810736774RNA PCR Primer, Index 1 (100% over 49bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC82380.10504521802620825RNA PCR Primer, Index 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)