FastQCFastQC Report
Fri 2 Aug 2024
HKMCHBGXV_n01_SRS_K562_WT_E1_Rep1_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMCHBGXV_n01_SRS_K562_WT_E1_Rep1_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6403979
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC691181.0792977303642002RNA PCR Primer, Index 10 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC409270.6390870426027319RNA PCR Primer, Index 10 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC263990.41222808507023523RNA PCR Primer, Index 10 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC248900.3886646099245485RNA PCR Primer, Index 10 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC233080.3639612184861943RNA PCR Primer, Index 10 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC218640.3414127372997319RNA PCR Primer, Index 10 (100% over 44bp)
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTAT195250.3048885700593334RNA PCR Primer, Index 10 (100% over 48bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGCGTATGC190060.2967842336772185RNA PCR Primer, Index 10 (98% over 50bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC176110.27500090178309455RNA PCR Primer, Index 10 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG173760.2713313082382063RNA PCR Primer, Index 10 (100% over 49bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC157290.24561292284062766RNA PCR Primer, Index 10 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTTTGC142380.22233052294518768RNA PCR Primer, Index 10 (98% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGC140530.21944169398431818RNA PCR Primer, Index 10 (97% over 44bp)
TAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC128580.20078142042626937RNA PCR Primer, Index 10 (100% over 44bp)
ACCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC123090.19220862529374316RNA PCR Primer, Index 10 (100% over 44bp)
CACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC122080.19063148083402523RNA PCR Primer, Index 10 (100% over 44bp)
AACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC120110.18755526837299122RNA PCR Primer, Index 10 (100% over 44bp)
CAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC111460.17404804106946634RNA PCR Primer, Index 10 (100% over 44bp)
TATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC110250.17215859077614087RNA PCR Primer, Index 10 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGC108180.16892622539830315RNA PCR Primer, Index 10 (97% over 44bp)
TTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC101140.15793306005531874RNA PCR Primer, Index 10 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGC99070.154700694677481RNA PCR Primer, Index 10 (97% over 44bp)
CTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC98540.15387308421842108RNA PCR Primer, Index 10 (100% over 44bp)
TCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC93650.1462372065867174RNA PCR Primer, Index 10 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGC91350.14264568950023104RNA PCR Primer, Index 10 (97% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGC88840.13872625128845675RNA PCR Primer, Index 10 (97% over 44bp)
CCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC85320.13322966861696453RNA PCR Primer, Index 10 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGCGTTTGC84890.1325582110747084RNA PCR Primer, Index 10 (97% over 46bp)
TTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC83580.1305126078645792RNA PCR Primer, Index 10 (100% over 44bp)
CATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC82950.12952884448871552RNA PCR Primer, Index 10 (100% over 44bp)
TCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC82930.12949761390535477RNA PCR Primer, Index 10 (100% over 44bp)
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGCGTAT81850.12781116240387422RNA PCR Primer, Index 10 (97% over 48bp)
TACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC80060.12501602519308697RNA PCR Primer, Index 10 (100% over 44bp)
ATCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC76480.11942575077151252RNA PCR Primer, Index 10 (100% over 44bp)
AGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC70860.11064995684714143RNA PCR Primer, Index 10 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGCGTATG70410.10994726872152455RNA PCR Primer, Index 10 (97% over 49bp)
CTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC70210.10963496288791702RNA PCR Primer, Index 10 (100% over 44bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG66960.10455999309179496RNA PCR Primer, Index 10 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)