FastQCFastQC Report
Fri 2 Aug 2024
HKMCHBGXV_n01_SRS_K562_WT_D1_Rep1_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMCHBGXV_n01_SRS_K562_WT_D1_Rep1_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8858822
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC1002371.1314935552379313RNA PCR Primer, Index 9 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC398450.4497776340917562RNA PCR Primer, Index 9 (100% over 44bp)
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTAT294860.3328433509556914RNA PCR Primer, Index 9 (100% over 48bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC271710.3067112083299563RNA PCR Primer, Index 9 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATG267780.3022749525839892RNA PCR Primer, Index 9 (100% over 49bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC256050.28903391444144605RNA PCR Primer, Index 9 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC233850.2639741491588837RNA PCR Primer, Index 9 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC231650.2614907489957468RNA PCR Primer, Index 9 (100% over 44bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC168660.19038648705211597RNA PCR Primer, Index 9 (100% over 44bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC165550.1868758622760453RNA PCR Primer, Index 9 (100% over 44bp)
TAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC139210.15714278941376178RNA PCR Primer, Index 9 (100% over 44bp)
CACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC121830.13752392812498096RNA PCR Primer, Index 9 (100% over 44bp)
TATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC118950.13427293154778366RNA PCR Primer, Index 9 (100% over 44bp)
ACCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC116740.13177824320208714RNA PCR Primer, Index 9 (100% over 44bp)
AACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC115710.13061556039843672RNA PCR Primer, Index 9 (100% over 44bp)
CAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC111810.12621316920014872RNA PCR Primer, Index 9 (100% over 44bp)
TTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC103970.1173632340733339RNA PCR Primer, Index 9 (100% over 44bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATG103940.11732936952565476RNA PCR Primer, Index 9 (100% over 49bp)
CTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC98820.11154982005508181RNA PCR Primer, Index 9 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)