FastQCFastQC Report
Fri 2 Aug 2024
HKMCHBGXV_n01_SRS_K562_WT_B1_Rep1_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMCHBGXV_n01_SRS_K562_WT_B1_Rep1_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10539388
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC322120.30563444480837026RNA PCR Primer, Index 8 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC281070.26668531417573776RNA PCR Primer, Index 8 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC176730.167685258385022RNA PCR Primer, Index 8 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC168980.16033189023878805RNA PCR Primer, Index 8 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC157600.14953429933502782RNA PCR Primer, Index 8 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC148360.1407671868613244RNA PCR Primer, Index 8 (100% over 44bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGC141610.13436264041137871RNA PCR Primer, Index 8 (97% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGTATGC124290.11792904863166628RNA PCR Primer, Index 8 (98% over 50bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC118830.11274848216993244RNA PCR Primer, Index 8 (100% over 44bp)
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTAT108870.1032982180749015RNA PCR Primer, Index 8 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)