FastQCFastQC Report
Fri 2 Aug 2024
HKMCHBGXV_n01_SRS_K562_WT_A1_Rep1_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMCHBGXV_n01_SRS_K562_WT_A1_Rep1_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10434113
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC498720.47797067177631675RNA PCR Primer, Index 7 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC318790.3055266892355872RNA PCR Primer, Index 7 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC203450.1949854290441363RNA PCR Primer, Index 7 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC191670.18369553789574639RNA PCR Primer, Index 7 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC181540.17398699822399855RNA PCR Primer, Index 7 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC168880.16185371962139952RNA PCR Primer, Index 7 (100% over 44bp)
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTAT139850.13403151758084275RNA PCR Primer, Index 7 (100% over 48bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC129650.12425589027069192RNA PCR Primer, Index 7 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATG127670.12235826849872145RNA PCR Primer, Index 7 (100% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCGCGTATGC112970.10826986443409228RNA PCR Primer, Index 7 (98% over 50bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC108580.10406251111139012RNA PCR Primer, Index 7 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)