FastQCFastQC Report
Tue 26 Nov 2019
HKLYGBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLYGBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29737166
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1670470.561744854906483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC42600.031.6789461
CAGACGT48200.028.6761823
TCGATCG28050.026.9240043
GCAGACG46100.026.7875582
TCCGGCT13750.025.907132
TTTCGTT72600.025.1305228
CGCGTAA78750.024.0759981
AGACGTT53050.023.4528624
CGATCGA29600.022.9416284
CGTAACT81600.022.759463
GGAGATG55150.022.0472872
CGTTCGA57700.021.8969027
TGTGGCG81650.021.0004167
GAAATGT85350.020.9803733
CATGTCG14350.020.72954664-65
CGGGAAC43550.020.6222321
GATCGAT33700.020.2902585
CTCCTGG35150.020.2646566
ATGGGCG60050.019.769716
CCTGGTG35500.019.5403068
Produced by FastQC (version 0.11.5)