FastQCFastQC Report
Tue 26 Nov 2019
HKLYGBGXC_n02_PACT_M4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLYGBGXC_n02_PACT_M4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50246823
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1494960.29752328818878754No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG1269750.2527025439996475No Hit
CCTCGATCGATTAGTACCAGTCAGCTCCAAACCTCACGGCTCTTCCACAC1207010.24021618242411066No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC1196120.23804888121981366No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1004860.19998478311753165No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG967270.19250371312033002No Hit
CGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTAC754860.15023039367085955No Hit
GGAAACTGCTGATCTAGAGTATCGGAGAGGAAAGTGGAATTCCTAGTGTA736790.1466341464016541No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGG736550.14658638218778527No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG732000.14568085229985586No Hit
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC709360.14117509479156523No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT693520.1380226566762241No Hit
CCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCA642260.12782101666407844No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG628640.12511039752702374No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG620890.12356801145417691No Hit
GCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCC613710.1221390653892685No Hit
GTTTACGGCCAGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT591730.11776465946911707No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA590410.11750195629283865No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT577320.1148968164614109No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC567230.11288872930334322No Hit
GGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCG563840.11221405978244635No Hit
CTCGCAGTCAAGCTCCCTTCTGCCTTTACACTCTTCGAGCGATTTCCGTC551260.1097104189054898No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA547490.10896012271263399No Hit
CTGGGCTACACACGTACTACAATGGGAGTTAATAGACGGAAGCGAGATCG531500.10577783196362484No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA519380.10336573916325019No Hit
CTTGGCGATATCAGCCGAAGAAGGACGCGGTAAGCTGCGATAAGCTACGG509170.10133376989824808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG213400.059.872213
CGATCGA217600.059.452224
CTCGATC236750.055.1436272
CGATTAG265700.048.1036348
ATCGATT319800.041.8481186
CTCCGAC89550.041.622021
GATTAGT339550.037.7677769
GCGTAAC158050.036.1909982
GATCCTG83000.035.360765
CTCGAGC73400.033.2686842
GGAAATG252400.030.9819722
ACCAGTC216450.030.53620716-17
AAATGTG254450.030.4418754
GAAATGT264700.030.0378553
TCACGGC217550.029.97612234-35
TCCGGCT31650.029.2704542
ATGTCGT231050.028.90529364-65
GTGTTTC413800.028.88229894-95
GGCAGAC67350.028.800181
GGCGCTT31900.028.7432062
Produced by FastQC (version 0.11.5)