FastQCFastQC Report
Tue 26 Nov 2019
HKLYGBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLYGBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29737166
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC38100.032.3809741
GCTTGAA135300.031.82064260-61
ATGCCGT138350.031.4823548-49
TATCTCG78850.031.0513540-41
CGATCGA28150.030.329454
TCGATCG31000.028.6124323
AAAAGGG131550.028.02928472-73
AGAGCAC535100.027.5836948
CTCGTAT137150.027.55960344-45
CAGACGT55300.027.5330773
GCAGACG49650.026.7432522
AAAGGGG130750.026.5976472-73
GGAGATG57950.025.8588452
CTTGAAA161100.025.52511662-63
CCGCTCA56900.025.23997332-33
CAGTCAC243950.025.10125426-27
ATCGATT37650.024.6944626
CGCGTAA84500.024.4272231
AAGGGGG147600.024.419274-75
AGGGGGG148700.024.23856274-75
Produced by FastQC (version 0.11.5)