FastQCFastQC Report
Tue 26 Nov 2019
HKLYGBGXC_n01_PACT_M9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLYGBGXC_n01_PACT_M9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53137085
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT6286521.1830758123069793TruSeq Adapter, Index 2 (97% over 37bp)
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG2357860.4437315294958314No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG2018810.37992486791475294No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGG1984060.373385179107962No Hit
CCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCA1851200.34838192573040094No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT1778660.33473044296652704No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1591030.29941988725952884No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1558870.2933676169853879No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1440460.27108374499654997No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC1430580.2692244032581012No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1408540.2650766409184847No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG1329780.2502546009063162No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG1322390.2488638584521526No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA1298270.24432465574654688No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC1281730.24121195206699803No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1185570.2231153628393428No Hit
GGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC1137810.21412728982028278No Hit
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC1117150.21023923310810144No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1101320.20726014609194315No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT1080970.20343042904969288No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGA1069720.20131326360864543No Hit
GCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCC1064160.20026691339955888No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1047480.19712786277229924No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG1027790.1934223527692571No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1016910.19137481854715968No Hit
GCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCT977060.18387534807376055No Hit
GCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT946400.1781053665250926No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG917820.17272682534241388No Hit
GGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT907870.17085431013010968No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG868380.16342258895082407No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA840860.15824353180081294No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT833860.1569261844152723No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA815550.15348038003966533No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG803450.15120325098751652No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT796130.14982568200720833No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC792500.1491425432915637No Hit
GGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACT783190.14739047126879468No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT777150.14625378866755676No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG777000.14622555979500945No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG763770.14373577323633768No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC761290.143269055876889No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA735550.13842498134777245No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA735060.13833276703078462No Hit
GGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGAC733420.13802413135760083No Hit
GGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGGC707430.1331330087075721No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG693530.13051713318485575No Hit
TCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGT672630.12658391027659874No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT661580.12450438333228102No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA654610.12319268172124986No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT651470.12260175732259307No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAA647370.12183016813963356No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG646010.12157422636187137No Hit
GCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAG635110.11952292829010099No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC626040.11781602246340762No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC625040.11762782997975896No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC603430.11356099040811139No Hit
GGCCAACCGAGGCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGA593680.11172611369253695No Hit
CACGAGCGTACGCGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAG579920.10913658511753138No Hit
CCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGT576480.10848920297378No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT571140.10748425511109615No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT570790.10741838774181911No Hit
GCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCG566050.10652635536932446No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA559460.10528616690207977No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG558270.10506221784653787No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA547310.10299962822574854No Hit
GCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGC546730.10289047658523234No Hit
TAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT545370.10263453480747016No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC542450.10208501275521607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG29200.045.2399331
GGCAGAC334450.044.2859151
TGCCGTC808700.042.41324650-51
TGCTTGA810000.042.12532460-61
GCAGACG356000.041.6135982
CTGCTTG780900.040.69656458-59
GGCGCTT35550.040.4830252
CTCACCC347250.040.432642
TATGCCG855750.040.11749348-49
CTCGTAT847550.040.02932744-45
ATCGAAG103200.039.9050376
GCCGTCT803250.039.82728250-51
GCTTGAA808900.039.587360-61
ATGCCGT815800.039.3689748-49
TCGAAGG98400.039.2450567
CGTATGC879750.039.17967646-47
AAAAGGG629250.038.89406272-73
CAGACGT427250.038.2312433
TCTTCTG840050.037.93840854-55
AAAAAGG694000.037.8101670-71
Produced by FastQC (version 0.11.5)