FastQCFastQC Report
Tue 26 Nov 2019
HKLYGBGXC_n01_PACT_M4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLYGBGXC_n01_PACT_M4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50246823
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3696230.735614667617891TruSeq Adapter, Index 7 (97% over 38bp)
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1514180.30134840564944776No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG1289290.2565913470787994No Hit
CCTCGATCGATTAGTACCAGTCAGCTCCAAACCTCACGGCTCTTCCACAC1238250.2464334909293668No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC1137380.22635858987542357No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1116180.22213941765034578No Hit
GGAAACTGCTGATCTAGAGTATCGGAGAGGAAAGTGGAATTCCTAGTGTA805830.16037431859124704No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG798590.15893343147287142No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGG775250.15428836167413013No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT745090.1482859921312836No Hit
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC730950.14547188386417984No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG653610.13007986594495735No Hit
GTTTACGGCCAGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT620980.12358592303437772No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG603790.12016481121602456No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC588520.11712581310862182No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA577250.11488288523236584No Hit
CCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCA575970.11462814275839887No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG569130.11326686266313793No Hit
GGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCG552580.10997312208176824No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA546950.10885265323142919No Hit
CGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTAC546150.10869343918519983No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA538190.10710925942521779No Hit
CTGATATCGACCGATAGCGCATAGTACCGTGAGGGAAAGGTGAAAAGAAC531680.10581365512402645No Hit
CTCGCAGTCAAGCTCCCTTCTGCCTTTACACTCTTCGAGCGATTTCCGTC527890.10505937858001489No Hit
CTTGGCGATATCAGCCGAAGAAGGACGCGGTAAGCTGCGATAAGCTACGG509930.10148502324216598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG202550.061.2977333
CGATCGA216150.058.4300044
CTCGATC231700.055.041772
CGATTAG265200.047.2134678
ATCGATT306200.043.26486
CTCCGAC76950.040.2638171
TGCCGTC514750.039.7322750-51
CTGCTTG490300.038.9914658-59
TATGCCG532750.038.41218648-49
ATGCCGT502650.038.41149548-49
TGCTTGA534000.038.059860-61
GCGTAAC152050.037.4547462
AAAAGGG407150.037.326672-73
GATTAGT336050.037.2033469
GCCGTCT520700.036.90203550-51
GTATGCC530150.036.67882546-47
TCTTCTG525450.036.57746554-55
CTTGAAA566650.036.1142962-63
GATCCTG81400.035.769535
CTCGAGC69400.035.5891842
Produced by FastQC (version 0.11.5)