FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9642939
Sequences flagged as poor quality0
Sequence length51
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC8550.020.78879744
GTCGCCC2350.020.10572645
GTCGCCG8400.019.01723945
TGCCGCA750.001106297118.0114611
CTCGCAT800.001708717716.8799652
CCCAGAT3600.016.87554
TGGTCGC10800.016.87444943
TCACATT2057.4578566E-1116.46270637
TCTCGGT23450.015.63914843
CCTCCTT2453.6379788E-1215.61214111
TTGTCAC2202.1827873E-1015.34024934
GCCAGAT8000.015.187954
CCCAGCT1209.761585E-515.0004454
GCCGGTT1056.044091E-414.99951245
CTCGGTG29600.014.89816344
CCAGATC12950.014.7687015
CACATTG2751.8189894E-1214.72679338
CCTACTT7350.014.6937810
CGCAGAT9950.014.4727924
TCCCGGG1251.382511E-414.39952945
Produced by FastQC (version 0.11.5)