FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n02_3LG_N-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n02_3LG_N-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208561
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC1650.036.8173744
GAACCCT250.002107379535.99920744
GTCGCCG1200.033.74925645
TGGTCGC1850.031.62092243
GTCGCCC501.982071E-631.49930645
GGCCGAA654.233122E-420.773072
CGACTAC706.984791E-419.28528810
GCCGAAA851.2001321E-418.5382061
GGTGGTC4100.018.10935641
GGGCGCC750.001110432617.99960344
CCGTGGT800.001711166916.87462840
CGGTGGT3100.016.69318240
CTGTGGG952.81998E-416.57858345
GCAGGAA3350.016.1270541
GGGCTGT850.002565645415.88200243
GCCGGAA1156.7555215E-515.6596021
CCTACTT1156.784244E-515.65182810
GTTCGAA1301.1015189E-515.5843161
TGGTAGC1451.8093288E-615.51689943
GGGTCGC1602.9640796E-715.46840943
Produced by FastQC (version 0.11.5)