FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n02_3LG_N-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n02_3LG_N-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498687
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG501.9780946E-631.50112345
GCCGGAA300.005136037730.0100981
GGTCGCC602.4135807E-730.00106844
TGGTCGC507.148674E-527.00096343
CGGTGGG2450.022.96000345
CCGACGT602.4625252E-422.50305710
CGAGCAT500.002262356722.5008037
CGCGGTG802.9682342E-622.50080144
GCAGGAA955.500933E-721.3229641
GCCAGAT654.2325768E-420.7699724
TCGCGGT654.2325768E-420.76997243
CGTCTTA550.00393036420.45732711
TGTACGT550.00393036420.4573279
CTTATAA550.003932647420.45527514
TAGGCGG908.233337E-620.00071344
TCTTATA600.006500344318.75254813
TATCGGT600.00650410918.75066843
TCGGTGG5100.018.0888845
CCAGATC1507.232302E-918.000645
AGGTTGG901.8624974E-417.50062445
Produced by FastQC (version 0.11.5)