FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n02_3LG_N-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n02_3LG_N-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356542
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAG352.8204947E-432.1431473
CTACTTT453.491089E-530.0002711
ACCCTCC300.005147355630.000279
CCCGAGC300.005147355630.000274
TACCTAT453.4919674E-529.99916323
CCCTCCT406.166396E-428.12525410
ACCTATC406.1677257E-428.12421624
ACGACTT507.158893E-527.00024410
CCTACTT901.1912562E-825.00022510
CCTCCTT450.001226604625.00022511
GTCGCCG1551.8189894E-1221.77358645
GCTACTT1257.530616E-1021.60019310
GACCTCT654.2385148E-420.76941912
CTTTTCT550.00393753320.45397630
CCTACGT908.252409E-620.0001810
TTTAAGT807.563755E-519.68767715
TGGTCGC1503.45608E-1019.49945643
ACCTACT2000.019.1251749
GGTCGCC1656.002665E-1119.09037644
ATTCGGA600.006491376618.759851
Produced by FastQC (version 0.11.5)