FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n02_3LG_N-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n02_3LG_N-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799772
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC601.546141E-1037.50078244
GGTGGCC250.002106510336.0007544
GTCGCCG708.1308826E-1032.1415245
GTCCTCT608.1901235E-626.24890712
CCGGCAG450.001224738925.0052132
CTCCTGT450.001226526224.99895911
GCAGGAA1450.024.8420831
TGGTCGC1001.3424142E-924.75051743
GCCAGAT751.6971844E-623.9989994
GTGGCCG500.002264560222.49906345
GTCGGCC500.002264560222.49906345
AGACTCC654.2378687E-420.7683669
GGCAGGC550.00393503120.45497143
CGCAGAT550.003936456520.4536934
GCCGGAA908.204592E-620.0116791
GATCCTC807.5617456E-519.6866810
TCAGGGG706.982884E-419.28491245
CCGAAAT600.006505671418.7515643
CAGTGGG750.001109666418.00037642
GCCTCCT750.001110132117.999259
Produced by FastQC (version 0.11.5)