FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n02_3LG_N-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n02_3LG_N-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862380
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAC207.0186536E-445.0177541
TCGTACT651.4028046E-831.15348410
ACCTACA300.00514684529.99965310
TCACATT406.165302E-428.12467437
GGTCGCC903.947207E-1027.4996844
GTCGCCG755.6363206E-826.99968745
CCCAGAT856.4774213E-926.4702824
CTTGAAT450.001226389424.99970814
CCAGATC2450.023.8772775
GCCGGAA500.00225897222.5088751
CTCGCTG500.002264307822.49973944
ACGGTGG2100.021.42832445
ACTCGGT754.6013323E-520.99975843
TCACGGT654.2373803E-420.7689943
CTCCGTG654.2373803E-420.7689944
AATCGCC654.2373803E-420.768997
CCCTCGT550.00393601820.45430810
TCCGTGG550.00393601820.45430845
TCTCAGT550.00393601820.45430843
GCGCTAC550.00393601820.4543087
Produced by FastQC (version 0.11.5)