FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n02_3LG_N-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n02_3LG_N-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104103
Sequences flagged as poor quality0
Sequence length51
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC352.8210052E-432.141545
GGCCGTA300.005131157630.0191251
GGACCGT300.005131157630.0191251
CCCAGAT801.0527583E-725.3148694
CGCTAAA450.001225207625.0046063
GTCCTAT450.001226502425.00007612
CCTTTAA551.3681268E-424.54552813
GGCCGAA851.88189E-723.8387171
GGTCGCC952.1162123E-823.68320844
AACCTCC500.002264514622.5000699
GTCGCCG903.2880416E-722.4990545
TGGTCGC1257.530616E-1021.59908743
ATTCGGA754.579877E-521.0133861
CGCAGAT1501.4551915E-1121.0019654
CCAGATC2150.020.9312445
GTCAGCA654.2198968E-420.782471
TCCTATA654.237999E-420.76929513
ACCTCCT600.00651022318.75005710
CTTTTAG851.2047766E-418.5286315
GCCGGAA1002.0353275E-518.0114731
Produced by FastQC (version 0.11.5)