FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n02_3LG_N-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n02_3LG_N-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212273
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCCT250.002107428435.99904310
CCGGATG300.00513662630.0128212
GTCGCCG903.965397E-1027.4992745
TCCGTGG450.001226783324.99933644
ACCCGGG551.3685095E-424.54480245
TGGTCGC1450.023.27524443
GCCGGAA1003.6203346E-822.5142611
GCCGCAG500.00225906522.5096172
CTCTGTG500.002265030322.49940343
ACGTACT702.7037055E-522.4994039
GGTCGCC1104.0545274E-922.49940144
GACGCAG1156.7357178E-921.5309352
ACCGGCA550.00392223920.467511
CTTGAAC550.003937265420.45400214
CGCAGAT1850.019.4589424
GGACGCA706.9540547E-419.297941
GCTCAGC600.00649465718.7580132
CTCGGTC600.00651169518.74950244
ACGCGGT600.00651169518.74950243
TCTCGGT4950.018.63586843
Produced by FastQC (version 0.11.5)