FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n02_3LG_N-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n02_3LG_N-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452894
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTTA250.002105153936.0021214
TGGACGC250.002106295435.99814643
GGTCGCC352.818547E-432.141244
CGGGCAA352.818547E-432.14121
GTAGCCG300.005145299229.99845345
AACGTCC406.158205E-428.1266579
GGGCCAG654.859903E-727.6939372
GGCCAGA755.6201316E-827.001593
ACGTCCT507.146115E-527.0015910
GCCAGAT901.186163E-825.0014724
CCAGATC1900.024.8698845
GCTCTCG1101.5097612E-1024.5441941
CTCTCGG1052.3592293E-923.57021342
TGCAGAT903.2759635E-722.5013264
TCTGGGT802.969482E-622.4988443
CTATCAC855.0185354E-621.1777225
CATTAGT855.0233393E-621.17537945
TCTCGGG1501.4551915E-1120.99891943
TGCATAG654.2310852E-420.7704522
CCTATCA654.2342403E-420.7681624
Produced by FastQC (version 0.11.5)