FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9642939
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCCC218600.035.4884426
GACCCCC79550.034.70303325
CGCCCCC61150.034.1808125
AACCCCC43350.033.7874125
ACACTCT628900.033.213781
TACCCCC46000.033.2104725
GCCCCCC191700.033.02720326
TCCGCCC22100.032.9823823
CACCCCC62400.032.48652325
GGCCCCC24750.032.36218625
CCGCCCC26000.032.1033324
GTGTTTC49600.031.567891
TGCCCCC32800.030.79892225
TGACCCC15350.030.63285324
CTCCCCC91100.030.50082825
ATGCCCC5950.030.24980224
CCCGCTT15100.029.7968962
TCGCCCC20700.029.2369124
GTACCCC7800.028.55552524
TAGCCCC13100.027.99405924
Produced by FastQC (version 0.11.5)