FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n01_3LG_N-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n01_3LG_N-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208561
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGT800.039.3727531
TGTGGCC250.002107312735.9994352
CCGGGTT1400.033.7494742
ACGTTCG401.5608004E-533.749475
ACACTCT108450.031.7409291
GTGTTTC5300.030.1397911
CCCGCTT2700.029.999532
GCCTTAA300.005151922329.99456810
TGGCCTT554.165675E-628.6359183
CCCGATT654.8744005E-727.6918752
TCCCGCT2200.027.6120621
CACGCTT5950.027.6046092
CCCTCTT15150.026.7322542
CCGCTTT2650.026.3203453
GGCCTTC608.192994E-626.249594
AAACTTT1900.026.0522233
CGGGTTT957.421477E-1026.0522233
AACTTTC2000.025.8745964
ACACTGT3850.025.712821
GACTCTT11450.025.5443951
Produced by FastQC (version 0.11.5)