FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n01_3LG_N-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n01_3LG_N-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498687
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTT207.036002E-444.9914782
CCGGTTT207.036002E-444.9914782
GTGGTTC502.1827873E-1144.9869651
TGTGTCC700.038.5641252
CACGGTC250.002107925735.9931834
ACGTTCC250.002107925735.9931835
GTGTTTC3250.035.989571
GGTGCCC250.002108963735.989571
TCACGCT850.034.4017941
CATCTGG401.5576115E-533.7537645
TTGTTTC551.1283919E-732.7177931
TGGGTCC352.821235E-432.136772
ACACTCT39600.031.5817571
GTCTTTC13450.030.270041
GGGCTTG300.005149263429.9943163
GGCCAGA300.005149263429.9943162
ATCCCTT300.005149263429.9943165
GTGTCCC751.7025741E-929.9943163
CTGTGTC453.493824E-529.991311
GACTCTT5200.029.8471181
Produced by FastQC (version 0.11.5)