FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n01_3LG_N-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n01_3LG_N-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356542
Sequences flagged as poor quality0
Sequence length51
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTT253.8937775E-544.994912
CCGTTAC250.002108793235.9946027
GTGTTTC6750.033.3295631
GTGTGTC1800.031.2464681
TCCCTCT5000.031.0464921
CCCGGGT1100.030.678351
ACACTCT109950.030.6718391
GTGTGGC1400.030.5322631
TCACTCT35800.030.0385041
CTTGGAT300.005149499529.99771525
TTTGACC300.00515042829.9966092
TGGTGCC300.00515042829.9966093
CGCTTTG1500.029.9966094
GACTCTT11750.029.8689631
CGGGTTT702.8901923E-828.92533
CACGCTT7250.028.2381862
TGGCGTT1700.027.7909763
TGGCTCC654.879312E-727.6891772
TTGTTTG903.965397E-1027.4968911
ACATTCG507.164846E-526.9969485
Produced by FastQC (version 0.11.5)