FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n01_3LG_N-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n01_3LG_N-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799772
Sequences flagged as poor quality0
Sequence length51
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGT700.041.7791521
CAGGCTT451.9297659E-839.993722
CCGGATT301.1406648E-437.4941142
TTTGCGG301.1406648E-437.4941141
CCGGGTT850.037.0530052
GTGTCCG458.706211E-734.9945073
CCCGCTT1050.034.2803342
GCGCGTT401.5616533E-533.74473
GTGTTTC4150.033.609181
CCCGGTT352.822214E-432.137811
ACACTCT77750.031.7409971
GACTCTT7800.030.8605381
CACGCTT4250.030.7010612
TCACTCT21650.030.6533831
CGGGTTT300.005150284629.9952933
CCGGCTT300.005150284629.9952932
CGGTTTG300.005150284629.9952933
GGTTTGC300.005150284629.9952934
ACGCATT453.493271E-529.995293
GTCCGTT453.493271E-529.995295
Produced by FastQC (version 0.11.5)