FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n01_3LG_N-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n01_3LG_N-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862380
Sequences flagged as poor quality0
Sequence length51
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTTT552.748493E-936.8169023
AGGGCCT250.002103580436.0112836
GCTTTGG250.002107176735.998755
ATACGCG250.002108976535.99248510
CCGGGTT754.7293724E-1132.998852
GTGTTTC3550.031.689041
GGGCTTT300.00514742829.9989593
GCCGGGT300.00514742829.9989591
TTTTCTC300.00514742829.9989591
TTTGGCG453.4906712E-529.9989572
GTGGTTC901.2732926E-1129.9989571
GCTTCGG300.005151808329.993749
ACACTCT70800.029.2044931
GACTCTT7800.029.1336021
TACCCGT406.166139E-428.1240225
CAGGCTT654.8724905E-727.6913432
GCATTCA507.158053E-526.9990625
TCACTCT24500.026.8153951
GTCTTTC17150.026.7629181
GTTTTCC608.190655E-626.2490881
Produced by FastQC (version 0.11.5)