FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n01_3LG_N-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n01_3LG_N-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104103
Sequences flagged as poor quality0
Sequence length51
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTT504.881258E-835.9978263
GTGTTTC5350.035.7455061
CCGGGTT702.1827873E-1135.3550072
GCATCGG1800.033.75866745
CCCGCTT1250.032.3980452
ACTTGCC352.8213055E-432.1409154
GTGTGTC2100.031.0695511
GCATGGG801.0004442E-1030.94544445
CCGCTTT1700.030.4393373
TGTTCGC453.4921584E-529.998191
GTTTACG300.005148692529.9981883
TCCCGGT300.005148692529.9981881
GGGCCAT300.005148692529.9981884
CCCGGTT300.005148692529.9981882
ACACTCT80050.029.7639751
GACTCTT10950.029.3817881
GTCTTTC24350.028.6430361
GCCGGGT406.168165E-428.1233021
CGGCTTT406.168165E-428.1233023
TACGCGG654.8853326E-727.68436410
Produced by FastQC (version 0.11.5)