FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n01_3LG_N-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n01_3LG_N-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212273
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGGCC207.0388097E-444.993031
CCCGGGT850.037.053081
CGGGTTG1150.035.213393
GTGTTTC7100.033.5863461
CCGGGTT1550.033.3819272
GGGGTTA352.823033E-432.137881
TCCCGCT1000.031.495121
ACACTCT99650.030.4769651
GAAAGGG300.005142962630.00525329
CGGGTAG300.005151269529.9953522
TGGCGCA453.494695E-529.9953521
GACTCTT13250.028.5238821
GTCTTTC28050.028.0705171
CACGCTT6500.027.6880172
GTGGTTC903.965397E-1027.4957391
CCCCTGT507.166271E-526.9958171
GCCGGGT507.166271E-526.9958171
CCCTGTT1250.026.9958172
GTATCTG3600.026.88081645
TCACTCT33100.026.5064681
Produced by FastQC (version 0.11.5)