FastQCFastQC Report
Fri 26 Apr 2019
HKLVVAFXY_n01_3LG_N-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLVVAFXY_n01_3LG_N-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452894
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCCC606.4301275E-933.7412223
GTGTTTC2500.031.4918081
CGTATGC651.3971658E-831.15950245
TGGAGTA300.005133884530.0120842
GGGTGTA300.00515054929.9921991
GTGTGTC751.7025741E-929.9921991
GCCCGAG300.00515054929.9921992
AGTGTGT300.00515054929.9921993
TGCCGGA300.00515054929.9921991
TGTGTCC852.0372681E-1029.1100752
GTCTTTC7750.028.734461
GCGTATG406.1458343E-428.13632444
TGTGTCA406.169706E-428.1176852
ACACTCT21300.027.7744641
GTGTGGC654.873473E-727.6851041
TTTTAGC507.161654E-526.9929771
TCACTCT8300.026.2883421
TGGCTAC608.193758E-626.2431744
TGTTTCC5100.026.022642
GACTCTT3400.025.8021131
Produced by FastQC (version 0.11.5)