FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41288959
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC2688850.055.6184168
CGGAAGA2920950.053.948854
GAGCACA2782400.053.337789
TCGGAAG2986700.053.1163143
ATCGGAA3070300.052.458632
GATCGGA3120250.051.808031
GAAGAGC3049850.050.0878456
AAGAGCA3056200.049.8317577
GGAAGAG3240250.047.8001175
CGTTCGA34400.033.14216232-33
CGTCTGA2181100.032.3827516-17
CACGTCT2229050.031.9360514-15
ACGTCTG2203650.031.64693314-15
GAACTCC2113950.031.60505120-21
ACACGTC2265750.031.35180512-13
CACACGT2331650.031.08481212-13
CTGAACT2209450.030.88099318-19
TGAACTC2242850.030.60102820-21
GCACACG2374650.030.47714410-11
AACTCCA2234050.029.81718422-23
Produced by FastQC (version 0.11.5)