FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64704042
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC987325315.259097723755804TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTATGC5465730.8447277528658875TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGGATGC981680.1517184969680874TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA17222800.063.2663579
AGAGCAC17683200.061.7588048
GAAGAGC18196000.060.3049856
AAGAGCA18325050.059.8023037
CGGAAGA18505050.059.715634
TCGGAAG18846150.058.82293
GGAAGAG18887200.058.2858735
ATCGGAA19245700.057.8199272
GATCGGA19572000.056.905771
CCGTCTT11364100.045.8009250-51
TGCCGTC11567800.045.61993848-49
CTCGTAT11041750.045.54084842-43
TATGCCG11641600.045.5091946-47
CGTATGC11691700.045.34787844-45
TGCTTGA10893550.045.33745658-59
ATCTCGT11222950.045.1166640-41
TCGTATG10945950.044.70966342-43
CGTCTTC11290000.044.70074550-51
GCCGTCT11377150.044.536848-49
ATGCCGT11552200.044.5102846-47
Produced by FastQC (version 0.11.5)