FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63167654
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC1102524117.453934572273337TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATGC6966761.102899911400857TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA16253250.074.5178459
AGAGCAC16612050.073.077588
CGGAAGA17074750.072.164344
GAAGAGC16943450.072.119846
TCGGAAG17293850.071.4892353
AAGAGCA17139550.071.1586467
ATCGGAA17516350.070.847852
GATCGGA17730050.070.1056061
GGAAGAG17521950.070.0206155
CTCGTAT12262100.045.82359742-43
CCGTCTT13002500.045.7474650-51
TGCCGTC13141200.045.67655648-49
TATGCCG13170850.045.6448746-47
CGTATGC13182450.045.589844-45
ATCTCGT12359900.045.55478740-41
TGCTTGA12687950.045.3073158-59
GGATCTC12604700.044.8335138-39
TCGTATG12218850.044.73451642-43
TCTCGTA12288300.044.6042640-41
GTCTTCT13042650.044.55434452-53
Produced by FastQC (version 0.11.5)