FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67211217
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC707835110.531502502030278TruSeq Adapter, Index 22 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTATGC3437080.5113848779140541TruSeq Adapter, Index 22 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA12976650.059.0446479
AGAGCAC13373550.057.3711558
GAAGAGC13811850.055.779716
CGGAAGA13982600.055.39814
AAGAGCA13968600.055.0929227
TCGGAAG14208950.054.650423
ATCGGAA14472850.053.795682
GGAAGAG14510000.053.2755435
GATCGGA14699650.052.9974631
CCGTCTT8125200.045.2118450-51
TGCCGTC8249950.044.9901248-49
CTCGTAT7943600.044.7981942-43
TATGCCG8306650.044.79750446-47
CGTCTTC8084750.044.63109650-51
CGTATGC8346850.044.62428744-45
GCCGTCT8130400.044.547548-49
TCGTATG7871400.044.45314842-43
ATGCCGT8252200.044.44594246-47
TGCTTGA7934050.044.28647258-59
ATCTCGT8081300.044.26943240-41
Produced by FastQC (version 0.11.5)