FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59311498
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC761542412.839709426998455TruSeq Adapter, Index 21 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCGCGTATGC4300310.7250381705078499TruSeq Adapter, Index 21 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA11013850.073.3577049
CGGAAGA11240100.072.758614
TCGGAAG11327250.072.3362053
AGAGCAC11205250.072.208498
ATCGGAA11413050.071.8788452
GAAGAGC11332750.071.6701666
GATCGGA11505350.071.351591
AAGAGCA11572200.070.130547
GGAAGAG11783650.069.103825
CCGTCTT8784800.045.6077650-51
TGCCGTC8854300.045.51366848-49
CTCGTAT8350950.045.4788342-43
TATGCCG8893400.045.38318346-47
CGTATGC8904400.045.29768844-45
ATCTCGT8426000.045.09386440-41
CGATCTC8418550.045.0933738-39
TCGTATG8330200.044.9795642-43
CGTCTTC8776200.044.8901450-51
GCCGTCT8813200.044.83099448-49
TCTCGTA8366250.044.81756240-41
Produced by FastQC (version 0.11.5)