FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53242514
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC544708310.230702103961507TruSeq Adapter, Index 20 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATGC3232380.6071050664512198TruSeq Adapter, Index 20 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA9066800.064.9680949
CGGAAGA9589500.062.270484
AGAGCAC9511350.062.0713548
TCGGAAG9714600.061.64463
GAAGAGC9657300.061.423696
ATCGGAA9810350.061.1761782
GATCGGA9948050.060.349541
AAGAGCA10016550.059.2069447
GGAAGAG10077900.059.034065
CCGTCTT6481800.045.25769450-51
CTCGTAT6115850.045.16924742-43
TGCCGTC6526600.045.13658548-49
TATGCCG6545750.045.0372146-47
CGTATGC6558300.044.90444-45
ATCTCGT6190550.044.5860840-41
TGCTTGA6518750.044.38664658-59
TCGTATG6100200.044.37080442-43
TCTCGTA6130100.044.15604840-41
CGTCTTC6483350.044.13216850-51
ATGCCGT6526300.044.06970646-47
Produced by FastQC (version 0.11.5)