FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58063882
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC32347695.571051897632335TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTCGTATGC21362473.6791322357674945TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATGC1394240.24012173350724292TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCGCGTATGC1140540.19642847855057297TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGTATGC719240.1238704639142109TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA10601450.056.2965779
AGAGCAC10748750.055.619648
GAAGAGC11036200.054.4290436
AAGAGCA11172750.053.70057
CGGAAGA11265300.053.677924
TCGGAAG11457550.052.893943
ATCGGAA11611650.052.3120542
GATCGGA11677550.052.0566751
GGAAGAG11666300.051.646855
CCGTCTT6406500.044.63010850-51
TGCCGTC6560900.044.16965548-49
TGCTTGA6158650.043.93045458-59
CGTCTTC6361850.043.7789850-51
CTCGTAT6176150.043.67597242-43
AAGGGGG6578850.043.633666-67
TATGCCG6662000.043.62516846-47
CGTATGC6468650.043.61905744-45
GCCGTCT6426050.043.53988348-49
GCTTGAA6282350.043.49114258-59
TCGTATG6116850.043.25935742-43
Produced by FastQC (version 0.11.5)