FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61351283
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC53961908.795561781487113TruSeq Adapter, Index 16 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCGCGTATGC3609820.5883854132276256TruSeq Adapter, Index 16 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA9560200.061.3699239
CGGAAGA10026650.059.1215064
TCGGAAG10145600.058.569983
ATCGGAA10217050.058.2398532
AGAGCAC10102700.058.2060628
GAAGAGC10168400.058.0273976
GATCGGA10299350.057.8028531
GGAAGAG10652250.055.5561565
AAGAGCA10639750.055.4589657
CTCGTAT5989550.045.2446642-43
CCGTCTT6433350.045.14929250-51
TGCCGTC6477200.045.03946748-49
TATGCCG6483200.045.03299346-47
CGTATGC6486750.044.93255244-45
TCGTATG5978650.044.85492742-43
TCTCGTA5997900.044.67054440-41
ATCTCGT6079050.044.55980740-41
ATGCCGT6466950.044.54176346-47
CGTCTTC6441950.044.47923750-51
GCCGTCT6467100.044.42895548-49
Produced by FastQC (version 0.11.5)