FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74194074
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC8070461.0877499461749465TruSeq Adapter, Index 15 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG1394400.030.44999566-67
GCTTGAA1442400.029.36339858-59
CGTCTTC1528950.027.86045650-51
CCGTCTT1566700.027.38470350-51
TGCTTGA1534800.027.24590358-59
AAAAGGG1583900.026.71446264-65
GCCGTCT1615450.026.4948248-49
TGCCGTC1711350.025.27239848-49
ATGCCGT1784450.024.193646-47
CTGCTTG1786650.023.46310256-57
TATGCCG1923900.022.56957246-47
TCGTATG1854550.021.92227642-43
CTCGTAT1929000.021.20173842-43
CGTATGC2061500.021.02524444-45
GTCTTCT2031550.020.92354452-53
GTATGCC2115150.020.52001244-45
TCTCGTA1995050.020.46314440-41
TCTGCTT2116100.019.986456-57
CTTCTGC2148950.019.84158954-55
TTCTGCT2122150.019.80008154-55
Produced by FastQC (version 0.11.5)